An analysis of enzyme action testing catalase activity

effect of ph on enzyme activity lab report

An enzyme is a biological catalyst. Account for any disparity between this figure and your calculated value.

enzyme activity lab report answers

During denturation the precise configuration of the active site is lost. As the temperature increases, the kinetic energy and thus the number of random collisions of enzyme with substrate increases per unit time. Since the data are almost linear, the rate is proportional to the concentration.

52 investigating catalase activity answers

A further increase in the temperature beyond the optimal temperature leads to disruption of the weak bonds of the enzyme - enzymes are proteins. At a concentration of 5 drops, the rate in the experiment should be about 0.

Enzyme activity lab answers

Optimum temperature is the temperature at which the enzyme has it maximum activity highest rate of reaction Q. The activity for the enzyme is highest at pH 10 and lowest at pH 4, since this is the ideal pH for the enzyme. Next, the group used a beaker with boiling water as a hot bath, and then lowered the temperature to about 55 degrees, then the test tube with the substrate and enzyme was placed into the hot bath. Account for any disparity between this figure and your calculated value. Calculate the temperature coefficient Q10 for your enzyme controlled reaction. Define optimum temperature. Discuss both high and low temperature effects. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? At a concentration of 5 drops, the rate in the experiment should be about 0. From your graph of activity against temperature, determine the optimum temperature. Predict what the rate would be for 5 drops.

Predict the the optimum temperature of a digestive enzyme such as pancreatic amylase. This is due to the temperature being ideal for the enzyme too high or too low temperatures cause denaturing. Firstly, once the substrate H2O2 was added to the enzyme oxygen bubbles were formed and led to an increased rate of reaction based on the amount of drops placed into the test tube with the enzyme.

Effect of enzyme concentration on enzyme activity lab report

What does this mean? What is the effect of enzyme concentration on enzyme activity? Since the data are almost linear, the rate is proportional to the concentration. Also, when placing the drops into the test tube, bubbles may have came out instead of the H2O2 causing there to be not enough dropped into the test tube. Another observation was that the color of the catalase was light brown and it was a liquid from potatoes. Another error may have been in temperature, when the hot water began to cool down and become room temperature. How does temperature affect the activity of catalase? Account for the decline in the slope after the optimal temperature is attained. Discuss both high and low temperature effects. Discuss the effect this variation might have on the functioning of enzymes in these organisms. Consider both high and low pH, and explain your observations by discussing the effect of pH on protein structure. The activity for the enzyme is highest at pH 10 and lowest at pH 4, since this is the ideal pH for the enzyme. Calculate the temperature coefficient Q10 for your enzyme controlled reaction. Usually, the enzyme activity increases from pH 4 to 10 when at low pH values, the protein may change its structure. At twenty-five drops, the reaction rate will most likely increase, since the rate doubles when the concentration of enzyme doubles.

Ectothermic organisms have body temperatures that vary with the temperature of their surroundings. Related Interests. Discuss both high and low temperature effects.

An analysis of enzyme action testing catalase activity

Also, when placing the drops into the test tube, bubbles may have came out instead of the H2O2 causing there to be not enough dropped into the test tube. Using what you have learned, what is the effect of substrate concentration on enzyme activity? In part two, the enzymes were heated, a clear decline in slope when placed in a hot bath was noticeable—most likely due to denaturing of the protein. Analysis: See graph in lab notebook Error Analysis: During the lab, a few errors may have occurred. The activity for the enzyme is highest at pH 10 and lowest at pH 4, since this is the ideal pH for the enzyme. Consider both high and low pH, and explain your observations by discussing the effect of pH on protein structure. How does enzyme activity change as substrate concentration decrease? Explain how enzyme activity changes as enzyme concentration, decreases, and discuss why this occurs on a molecular level. An enzyme is a biological catalyst. In this lab several things were observed. Since pancreatic amylase is a digestive enzyme its optimum temperature would be boy temperature ie.

The unfolding of the protein due to the disruption of bonds is called denaturing.

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Effect of Temperature on Enzyme Action